In vitro ANTIFUNGAL ACTIVITY OF THE AQUEOUS AND ALKALOID EXTRACT OF Lupinus spp. ON Moniliophthora roreri

Abstract

An evaluation of the in vitro antifungal activity of aqueous and alkaloid extracts of seeds, leaves and stems
of L. campestris (Lc) and L. montanus (Lm) on the inhibition of the mycelial growth and sporulation of
M. roreri was performed. The lower mycelial growth (p?0.05) of M. roreri was recorded at 25 % of the
aqueous extract of Lm seeds. Similarly, the total alkaloid extracts from seeds and leaves plus stems of Lc and Lm inhibited the mycelial growth of M. roreri, showing a total inhibition in all the concentrations up to six days of evaluation, after which the fungus growth was activated, although without reaching the control. Sporulation was only affected by the extract of alkaloids from seeds. The concentration of 10 mg mL^-1 presented the highest sporulation inhibition (83.6 %) in Lm seeds. The levels of inhibition of mycelial growth with Lm seed extracts were 84.8 % to 93.6 % (aqueous) and 73.4% to 85.2% (alkaloid); and the extracts of alkaloids from Lm leaves plus stems were 61.3% to 79.7%, and for Lc, 57.9% to 72.1%.