In vitro AND ex situ COLLECTION AND CONSERVATION OF Carica papaya L. PHYTOGENETIC RESOURCES

Abstract

Fruit buds from Creole papaya (Carica papaya L.) were collected in the center and south of the state of Baja California Sur, México, in the localities of El Pescadero, Ejido en Comondú Ley Federal de Aguas #2, La Paz, La Purísima, La Ribera, San Bartolo, San Javier, San Miguel de Comondú and Todos Santos, with the aim of conservation and multiplication. The buds were multiplied under aseptic conditions, in glass containers with 20 ml of Murashige-Skoog (MS) (1962) medium, with 3 % sucrose and 0.8 % agar for its in vitro cultivation. Treatments were used for the in vitro propagation and rooting of the buds; the results obtained show that 2 mg L^-1 of naphthalene acetic acid increase the production of buds. The indole-butyric acid at a concentration of 3 uM generates the rooting of the buds, which once established develop quickly. Part of this material was transferred to greenhouse conditions for its acclimation and later planting into the field. Therefore, germplasm from Creole papaya seeds and plants is conserved in vitro and ex situ.