In vitro CONSERVATION FROM MINIMUM GROWTH OF Swietenia macrophylla King AND Tectona grandis L.

Abstract

With the objective of conserving in vitro the species Tectona grandis L. and Swietenia macrophylla King, through the technique of minimum growth under in vitro conditions, the mannitol concentration was evaluated (10 g L^-1, 15 g L^-1, 20 g L^-1 and 30 g L^-1), as well as the application of a layer of mineral oil (2 ml) in combination with three temperatures (18 °C, 24 °C, 28 °C). In T. grandis, the mineral oil reduced growth significantly, but it decreased the number of buds and the vigor, while in S. macrophylla, it increased growth and decreased the number of buds and vigor of the explant. With regard to the use of mannitol, T. grandis and S. macrophylla showed that the application of 15 g L^-1 to the cultivation medium reduced in vitro growth significantly, vigor and survival were maintained, and the conservation temperature of 18 °C generated a synergetic response and reduced in vitro growth with greater efficiency in both species up to fifteen weeks.