Induction of shoot development of Stevia rebaudiana Morrita II by vitamins and cysteine

Abstract

ABSTRACT

Objective: The objective of this work was to evaluate the influence of B vitamins and cysteine ​​on the formation and development of shoots of Stevia rebaudiana Morita II, without the addition of plant growth regulators.

Design/methodology/approach: The starting material was nodal segments, using eight treatments of different vitamins, the results were analyzed with a comparison of means and a Tukey test to show statistical differences.

Results: The best treatment was number two with an average of 7.5 shoots/explant, showing that it is efficient for multiplication; In the case of explant elongation, treatment five turned out to be the most efficient.

Limitations of the study/implications: The implications of the study are to obtain a multiplication protocol with better efficiency; within the limitations of this work is its transfer to temporary immersion bioreactors.

Findings/conclusions: Thiamin and cysteine ​​are two organic compounds that induce multiple sprouting, while nicotinic acid and pyridoxine stimulate the elongation of Stevia explants, taking another step to transfer this knowledge to grassroots companies technological.