Molecular identification by PCR of the farm worm in the Chontalpa region, Tabasco, México
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Keywords
Spodoptera frugiperda, rice, barcoding, pests
Abstract
Goal. – Carry out the first molecular characterization of the Bardcoding COI region of S. frugiperda in Tabasco, as well as test whether it can reliably identify the race of S. frugiperda present.
Design/methodology/approach. - Specimens of S. frugiperda were collected using four pheromone traps in maize plots. DNA extraction, PCR with LCOL/HCOL primers, and sequencing of six individuals captured in traps were performed.
Results. - The sequences were aligned and a phylogenetic tree was built, which groups the S. frugiperda of this study in the rice race.
Implications. - Identification of the race of S. frugiperda is necessary for management purposes, since each race may require a different control method.
Findings/conclusions. - Our study revealed that the isolates from Chontalpa, Tabasco in Mexico correspond to races of fall armyworm reported in other latitudes belonging to the RS. This becomes the first report of this race of S. frugiperda in Tabasco, relevant since this species has the potential to become a serious pest in the sugarcane and rice areas of the state.