Physiological development of anthurium (Anthurium andreanum Linden) var. Tropical red color in three in vitro culture systems

Abstract

ABSTRACT

Objective: to evaluate the physiological development of Anthurium andreanum L. var. Tropical red color in three in vitro culture systems: semi-solid, partial immersion and RITA® bioreactor

Design / methodology / approach: A completely randomized design was used with three treatments: semi-solid medium, partial immersion and RITA® bioreactor, with four repetitions each. Vitroplants of anthurium were selected with a size of 0.5 cm from the stem to the highest leaf, with three leaves each specimen. After 60 days of sowing, morphometric analyzes, chlorophyll content and hormone content were carried out. An analysis of variance and comparison tests of means were performed on the data obtained by Kruskal-Wallis and Tukey, respectively, using the statistical software R-STUDIO.

Results: The highest shoot rate and root length was obtained in partial immersion; however, the number of leaves, shoots and root multiplication did not show differences with the RITA® bioreactors. The highest concentration of chlorophyll and indole acetic acid was observed when using RITA® bioreactors.

Study limitations / implications: The results are favorable for the in vitro production of anthurium, however for commercial production the use of RITA® bioreactors is a high cost in the initial investment.

Findings / conclusions: with the results obtained, it is considered that the RITA bioreactors obtained the best results for the production of anthurium, followed by the partial immersion system. Due to the liquid medium and better gas exchange, which favors the development of plants

Keywords: Anthurium andreanum L.; chlorophyll; phytohormones; immersion systems