QUANTIFICATION OF FLAVONOIDS IN Acer negundo EXTRACTS BY HPLC ANALYSIS
Main Article Content
Keywords
ferulic acid, gentisic acid, flavonoids, HPLC.
Abstract
- a) objective: The objective of this work was to identify and quantify flavonoids from leaf and stem extracts of Acer negundo by High-Performance Liquid Chromatography.
- b) design / methodology / approach: Ethanolic extracts of Acer negundo were subjected to High-Performance Liquid Chromatography for the quantification and identification of the main antioxidant flavonoids.
- c) results: In leaf extracts the highest concentrations were for rutin (34.19 µg/mL) and catechin (33.97 µg/mL); in mean concentration apigenin (19.05 µg/mL), gallic acid (19. 04 µg/mL), ferulic acid (17.2 µg/mL) and 2.5 dihydroxybenzoic acid (12.72 µg/mL); and in lower concentration caffeic acid (6.15 µg/mL), quercetin-3-?-glucoside (4.97 µg/mL) and isorhamnetin (4.68 µg/mL). In the stem extracts the highest concentrations were for ferulic acid (7.96 µg/mL), rutin (5.61 µg/mL) and catechin (4.37 µg/mL); at the medium concentration isorhamnetin (3.31 µg/mL) and quercetin-3-?-glucoside (2.01 µg/mL) were identified and at the lowest concentration apigenin (0.79 µg/mL) was identified but gallic acid, caffeic acid and 2,5-dihydroxybenzoic acid were not detected.
- d) limitations/implications: Some flavonoids have been identified in other Acer species, but have not been identified and quantified in Acer negundo, a Mexican species.
- e) findings/conclusions: For the first time we report on gentisic acid in Acer negundo leaf extracts. This analytical method can be standardized to serve as a quality analysis of maple tree products.