EFFECT OF GROWTH REGULATORS ON THE in vitro REPRODUCTION OF Musa sp. CV GRAN ENANO

Abstract

The study was carried out in the Biotechnology Laboratory of the Agricultural Sciences School Campus IV (UNACH) with the objective of determining the effect of two traditional growth regulators and one homobrassinolide (brassinosteroid) added in two concentrations to the MS medium for the in vitro growth of Gran Enano banana (Musa sp.). It was established on two growth media based on modified Murashige and Skoog (MS) (1962), and three growth regulators, BAP, AIA and Br in two concentrations of 2 mg L^-1 and 4 mg L^-1, at pH of 5.6. The explants were incubated at 26±1 °C, 60% H.R., I.L: 45 mE.m^-2 s^-1 and a light period of 16 hours light and 8 darkness; in total, 14 treatments with 10 repetitions in a completely random design. The number of buds, leaves, roots and height were measured. The results indicated differential effect between growth media and concentrations of growth regulators. In the radical induction, the MS medium at 50 % in interaction with AIA and brassinosteroid increased the number of roots in the explants, while the number of leaves increased with BAP and brassinosteroid and decreased with AIA.