Morphological characters and identification of sub-phases during imbibition in seeds of stock (Matthiola incana) Germinatión in stock

Abstract

Objective: To describe morphologic traits and imbibition stages in seeds from three accessions of Matthiola incana.

Design/methodology/approximation: Seeds were analysed from Nieve, Rosita and Uva genotipes, which correspond to the genus Matthiola and species incana. In the first assessment, weight and colour was determined in a thousand seeds. The area, perimeter, length (major axis), width, centric angle, elongation (length/width) and round degree were obtained by means of processing digital images. Analysis of variance was performed to the data followed by a multiple comparison of means by the Tukey procedure. For the imbibition test, fifty seeds of each genotype were immersed in water and evaluated every 24, 48, 72 and 164 hours in order to know the absorption dynamic and determine the time of the imbibition sub and phases. 

Results: Physical description in Nieve seeds show a moderate orange colour and thousand seeds weight 0.218 g; Rosita seeds have a moderate orange yellowish colour and thousand seeds weight 0.1902 g; Uva seeds show a colour from greyish to dark brown with a weight of 0.1272 in thousand seeds. Nieve seeds show greater morphologic dimensions than Rosita and Uva seeds, but the three genotypes show a similar trait in the form type. During the imbibition test of each genotype follow a progressive rate until stage three, where the imbibition behaviour is synchronised with the radical protrude emergency to 164 h after the test was initiated. 

Study limitations/implications: Seeds show a different size, therefore great samples are difficult to handle at the imbibition moment.

Findings/conclusions: It is determined from the three accessions that seeds from Matthiola incana species have greater morphologic traits than other species from the same genus. Two phases were identified during the phase two, the first one was known as the moment of solute explosion and the second one was known as the recovery of lost solutes.