DIRECT ORGANOGENESIS OF FLORAL BUDS AND SOMACLONAL VARIATION OF Musa sp. EVALUATED BY MOLECULAR MARKERS

Abstract

In this study the somaclonal variation of plants regenerated in vitro through direct organogenesis (DO), from floral bud explants of Musa sp. in proliferation, was researched, by using inter simple sequence repeats (ISSR), sequence repeats amplified polymorphism (SRAP), and intron directed amplified polymorphism (ITAP) markers. The immature masculine flowers of M. a. X M. b. (AAB, Silk) cv. Manzano served as initial explant for the in vitro regeneration of plants through DO. The formation of floral buds in proliferation was in average 5.9 buds explant^-1. These buds were used to induce the differentiation of buds via DO. 4.4 buds were formed per explant. The use of ISSR starters, SRAP combinations and ITAP
combinations revealed polymorphism between plants regenerated through DO of 36.3, 19.5 and 13.7%, respectively. In this study, the factors evaluated for the in vitro regeneration via DO and its influence in the variation observed are discussed.