Serological response of lambs vaccinated with a biological bacterin-toxoid type against caseous lymphadenitis

Abstract

Objective: To evaluate the serological response to somatic, secretory, and soluble antigens in sheep vaccinated and revaccinated with an experimental bacterin-toxoid biological against ovine caseous lymphadenitis. Design/Methodology/Approach: The serological response to different antigens was assessed in sheep vaccinated with an experimental bacterin-toxoid biological against caseous lymphadenitis. The animals’ sera were tested using indirect enzyme-linked immunosorbent assay (ELISA) with different antigens. The antigens were obtained from a wild strain isolated from an ovine abscess. Results: The antigens with the strongest immunogenic response were those obtained by sonication and the supernatant rich in phospholipase D.

Study Limitations/Implications: In Mexico, no commercial vaccines are currently available for the prevention of caseous lymphadenitis. Therefore, conducting research to develop effective control strategies for this disease is of great importance. Findings/Conclusions: The most effective antigens were those obtained by sonication and the supernatant rich in phospholipase D, which is significant since phospholipase D is the primary pathogenic factor of Corynebacterium pseudotuberculosis. Vaccination against caseous lymphadenitis using a bacterin-toxoid biological could serve as a promising alternative for disease prevention, given the strong antigenic response observed in this study.