Acondicionamiento y evaluación de tratamientos de desinfección para la micropropagación de Sagittaria macrophylla zucc. procedente de las Ciénegas del Rio Lerma
Main Article Content
Keywords
water potato, danger of extinction, substrate, disinfection, in vitro culture.
Abstract
Objective: To evaluate the adaptability and a disinfection method of Sagittaria macrophylla zucc. (Alismataceae) from the Cienega de Lerma, for its propagation and conservation purposes.
Design / methodology / approach: In a greenhouse, a completely randomized block design was established to evaluate two types of substrates for S. macrophylla, and the number and length of shoots were evaluated. The first substrate consisted of a mixture of black soil and agrellite with a ratio of 2:1, respectively (S1); the second substrate was made of sediments from the collection site located at the Ciénega del Rio Lerma (S2) and considered the control. To evaluate an adequate disinfection method for shoots multiplication in vitro, a randomized complete block design was established in which the treatments consisted of several washes + 70% ethanol + commercial chlorine (T1) and washes + microdin-liquid soap + 70% ethanol + commercial chlorine (T2). The photoperiod conditions (light and dark) represented the blocks of the experimental design.
Results: In the substrate experiment, significant statistical differences (P<0.05) were observed between the treatments where S1 showed 2.4 ± 0.24 shoots on average with a length of 2.5 cm in 30 days, showing a trend to increase. In the disinfection experiment, significant statistical differences (P<0.05) were observed between the treatments, however the photoperiod was not significant (P>0.05). The T2 presented the lowest percentage of contamination 31.25%.
Study limitations / implications: The results presented here are advances of a long-term experiment.
Findings / Conclusions: The conditioning of S. macrophylla was favorable under greenhouse conditions in black soil and agrellite substrate (2:1), allowing new and healthy shoots. The best disinfection method consisted of rinsing with tap water, powder soap, 30 minutes with 100 ml of distilled water plus one drop of commercial microdin and two drops of liquid soap, two minutes in constant agitation in 70% ethanol and 20 minutes in commercial chlorine (6%) at 30% v / v, using the axillary buds as explants, regardless of the photoperiod in the incubation room.
