Solid lipid nanoparticles loaded with Jackfruit (Artocarpus hetero-phyllus Lam) seed-derived bioactive peptides: Characterization and antioxidant activity.

Resumen

Objective: Characterize and evaluate the antioxidant activity of solid lipid nanoparticles loaded with bioactive peptides derived from the cotyledon of jackfruit

Design/methodology/approach: The peptides were obtained from seed cotyledon by ultrasound-assited sequencial enzymatic hydrolysis. Four peptide fractions (PF) of ≥ 10 kDa, ≤ 10 kDa, ≥5 kDa, and ≤5 kDa, and their antioxidant activities were evaluated by using the DPPH radical scavenging method. A double emulsion method encapsulated PF≥ 5 kDa and ≤ 5 kDa into solid lipid nanoparticles (SLN).

Results: The UA-AF 2 treatment resulted in higher HD (40.39 ± 2.82 %). Besides, the effect of ultrasound improved the antioxidant activity on the DPPH. The SLN had a particle size (Ps) mean of 235 nm, polydispersity index < of 0.4, and negative zeta potential (ζ) > -30 mV and the SLNs were considered stables. The antioxidant activities of the peptide fractions in SLN decreased by 67.65 ± 3.19% and 52.21 ± 1.15%, associated with higher encapsulation efficiencies of peptide fraction in SLNs, ≥ 5 kDa (84.43 ± 7.44 %) and ≤ 5 kDa (81.68 ± 8.31 %).

Study limitations/implications: SLN loaded with Jackfruit (Artocarpus heterophyllus Lam) seed-derived bioactive peptides could have potential applications in food preservation.

Conclusions: Peptide fractions were effectively entrapped in SLN prepared by double-emulsification. These SLN were nanometric size, with good encapsulation efficiency, and maintained antioxidant activity