In vitro Anthelmintic activity of Musa balbisiana Colla (Square banana) against Haemonchus contortus eggs IN VITRO ANTHELMINTIC ACTIVITY OF MUSA BALBISIANA COLLA (SQUARE BANANA) AGAINST HAEMONCHUS CONTORTUS EGG

Resumen

Objective: To evaluate the anthelmintic activity of aqueous and ethanolic extracts of Musa balbisiana Colla, against H. contortus eggs.

Design / methodology / approach: The anthelmintic activity was evaluated using an egg hatching inhibition test. The aqueous extracts from leaves, peels and roots were obtained by infusion and subsequently lyophilized. Ethanolic extracts were obtained by maceration and later concentrated in a rotary evaporator. Spectroscopic, phytochemical, chemical and total polyphenol content analyzes were performed. The 50 % lethal concentration to inhibit H. contortus eggs from hatching was calculated following a Probit analysis.

Results: The identified functional groups in the FT-IR analysis were hydroxyl (-OH) and methyl groups (CH3). The proximal analysis revealed significant differences in the dry matter percentage (P < 0.05). No significant differences were found in the protein content (P > 0.05). The egg hatching inhibition rates at the highest concentration 4.8 mg / mL were 100 % for the aqueous and ethanolic extracts from leaves, and 93.7 and 62 % for the shell and roots, respectively.

Study limitations / implications: Further studies are required in in vivo systems.

Findings / Conclusions: With a LC50 of 225 µg / mL and a 95 % confidence interval, with a range between 33 and 418.4 µg / mL, the aqueous extract from the leaves was the most active.