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Abstract
Objective: To isolate and carry out a molecular characterization of microorganisms potentially tolerant to high concentrations of different pesticides (glyphosate, carbofuran, and chlorpyrifos).
Design/Methodology/Approach: Based on the isolate project SIP20170193, only the strain GVE 5 was chosen for the experiment, as a result of its morphological and growth characteristics. The tolerance capacity (TC) of GVE 5 to three different pesticides (glyphosate, carbofuran, and chlorpyrifos) was evaluated in two different media (LB and M9). The only carbon source was 200 mg/L of each pesticide. GVE 5 was identified through the Polymerase Chain Reaction (PCR) molecular techniques and amplified by the 16S rRNA marker.
Results: Based on the TC analysis, the GVE 5 strain of Priestia aryabhattai recorded a growth with 200 mg L-1 of glyphosate, carbofuran, and chlorpyrifos in LB medium and M9 minimal medium.
Study Limitations/Implications: There were no limitations or implications for this study.
Findings/Conclusions: Priestia aryabhattai is tolerant to 200 mg L-1 of glyphosate, carbofuran, and chlorpyrifos. These results open new lines of research regarding the bioremediation of soils polluted by these agrochemicals. Priestia aryabhattai should be subjected to further evaluations as a plant growth promoter.