Development of xylophagous fungi native to Veracruz in sugarcane (Sacharum spp.) distillery vinasses on inert materials of zeolite and agricultural lime

Resumen

Objective: To establish the development conditions of white-rot fungi with the use of vinasse as substrate and zeolite and commercial agricultural lime as support.         

Design/methodology/approach: Pure vinasse from a sugarcane distillery was used as the main nutrient on a support of vinnase-zeolite and vinasse-lime in 1:1 proportion. The variable of study was the daily radial growth (mm-day^-1) of each of the four isolates on vinasse-inert culture medium. The experimental unit was glass Petri dishes of 90x15 mm by triplicate. A Mann-Whitney U test was conducted (using R Studio).

Results: Four fungal isolates (G = Ganoderma sp. K., P=Pleurotus sp. B., Py= Pycnoporus sp. K., and Tr= Trametes sp. C.) were cultured on zeolite-vinasse and agricultural lime-vinasse and their daily radial growth was recorded. Two fungal isolates were able to develop tissue covering the Petri dish in 9 days. Zeolite-vinasse was the best treatment, showing a median of 2.1 mm-day^-1 on isolate G and 1.6 mm-day^-1 on isolate P with significant differences (p< 0.05).

Limitations on study/implications: All the white-rot fungi strains test were cultured at 28°C and minimal development conditions with the use of a byproduct of the sugarcane industry and the use of non-conventional inerts.

Findings/conclusions: The development of mycelium by the isolated fungi G and P on a substrate with zeolite-vinasse recorded the highest growth compared to the agricultural lime.